Introducing the PFAFF® creative icon™ sewing and embroidery machine. It’s where perfection meets freedom. Mission Impossible S02 - Ep07 Operation Heart HD Watch. The alignment was visualized with Pfaat 2.0.117, highlighting residues with ≥50% conservation 46. The EF-hand motifs are denoted by solid (α-helices) and dotted (N-terminal S100-and Cterminal.
Software for exploring PTP sequence
and structure files at http://ptp.cshl.edu and http://science.novonordisk.com/ptp
Genedoc - version 2.6.02
An excellent multiple sequence alignment viewer, editor, and analyzer with advanced shading utility for data visualization, paginated printouts and figure export. In addition, the alignment information can be applied to 3D graphics using structure information from the Protein Data Bank (PDB).
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Pfast Chemicals
Pfaat (Protein Family Alignment Annotation Tool)
Pfaat is a Java-based protein sequence alignment application designed to facilitate the analysis, curation, and annotation of large protein sequence families. Key features of Pfaat include the ability to align collections of sequences, group sequences into specific families, analyze sequences based on a number of similarity criteria, and annotate sequences and specific residue positions with text descriptions. For Mac users, Pfaat is a good alternative to the Genedoc alignment viewer.
ClustalX - version 1.81 (Mac OS X Version, click here)
This program provides an integrated window-based environment for performing multiple sequence alignments and for calculating homology trees. Trees are calculated from imported or newly generated alignments and are easily bootstrapped to derive confidence values for the groupings in a tree. Specifically, the pulldown menus at the top of the window allow you to load alignment files (*.aln or *.msf) and select options required to add new sequences to the alignment, change the order of sequences in the alignment and realign a sub-range of the alignment. The Tree menu allows you to calculate Phylip format tree files (*.ph) based on the loaded alignment. Subsequently you can bootstrap the generated tree (*.phb) and view this tree using the program Treeview (see below).
Treeview - version 1.6.6 (Mac OS X Version, click here)
This is an easy-to-use program for viewing and printing tree files. The program reads the phylip format tree (*.ph or *.phb) calculated by ClustalX and visualizes bootstrap labels at the node of the tree. When using ClustalX to bootstrap trees remember to change the default output format option for boostrap labels from branch to node (e.g. in the Tree pull-down menu in ClustalX select 'output format options' and choose node instead of branch for bootstrap labels). In the Tree pull-down menu in Treeview choose show internal edge labels if you are viewing a bootstrapped tree.
Swiss-PdbViewer - version 3.7
The Swiss-PdbViewer provides a user-friendly interface that allows you to view and analyse several protein structures at the same time. The proteins can be superimposed in order to compare their active sites or any other relevant parts. Amino acid mutations, H-bonds, angles and distances between atoms are easy to obtain using the menu interface. In addition, several basic modeling tools are available.
PyMOL - version 0.98
PyMOL is an open source molecular viewer and modeling system supported on Windows, Mac and Linux. The program has outstanding molecular representations (lines, sticks, dots, mesh, spheres, ribbons, cartoons, and surfaces) and has a built-in photorealistic ray tracer that allows for generation of high-quality molecular graphics images and animations. The program can be run interactively (using menu-driven commands and options) or via input scripts, but most efficiently by a combination of both approaches. All manipulations can be saved to a log file (*.pml) and the generation of command scripts (via automatic logging) allows the user to save favorite views and representations of any Protein Data Bank struture file (*.pdb) and to reuse these scripts for viewing other structures. Of note, PyMOL must be installed on your computer prior to running a script (*.pml) or session file (*.pse). All scripts have the file extension *.pml and are executed from the File Menu by selecting run and locating the script file on your hard drive. The PyMOL scripts from our website require that the appropiate PDB file is loaded. To find the appropiate PDB file, use a text editor to view the instructions in the heading of the *.pml file. (In contrast, PyMOL session files with the extensiion *.pse do not require a preloaded PDB file). To read more about this excellent program and its user-sponsored concept go to http://pymol.sourceforge.net/
Vector NTI
A highly integrated and comprehensive software package for desktop sequence analysis and molecular biology data management. Provides a powerful collection of sequence analysis software in one program. For details on this liciensed software see http://www.informaxinc.com/
Pfastbac Dual
6/15/16 - Max FB: 86, FB: 83-86, CB: 71-75
6-foot-2, 190-pound RHP withan athletic frame, arm action is loose and quick, works through a ¾ slot that produces good arm-side run on his fastball, breaking ball is very sharp with 11/5 action, had a tendency to miss out (to RHH’s) with the breaking ball in this outing but it has potential to be a college level swing and miss pitch, allowed one hit, walked two, and struck out two in his two innings of work. Pfaadts frame and quick arm should lend to more velocity development. Would not be surprised at all to see him consistently in the high-80s his senior season.